Abcam, ab316072, Recombinant Human CD20 VLP (Active) protein

Size: 100µg / 500µg
Recombinant Human CD20 VLP (Active) protein is a Human Full Length protein, in the 1 to 297 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for ELISA and HPLC. The predicted molecular weight of ab316072 protein is 34.3 kDa. - Save time and ensure accurate results - use our CD20 VLP (virus like particles) protein as a control - Optimal protein bioactivity, stability and reproducibility - Available in different sizes to fit your experimental needs
Key facts
Purity:>95% HPLC,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:ELISA, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Immobilized Human CD20 VLP at 5ug/ml (100ul/Well) on plate on the plate. Dose response curve for Ofatumumab, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.,
Accession:P11836,
Animal free:No,
Carrier free:Yes,
Species:Human,
Storage buffer:Constituents: PBS

Product details:
Ensure the validity of your result using our bioactive recombinant human CD20 VLP (virus like particles) protein ab316072 as a positive control in HPLC.
Analyze your CD20 VLP ELISA data using the ab316072protein to generate and plot a standard curve.
Check out our ELISA protocol for more information

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.