Abcam, ab316090, Recombinant Human IL-7 (Active) protein

Size: 100µg / 500µg
Recombinant Human IL-7 (Active) protein is a Human Fragment protein, in the 26 to 177 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level.
Key facts
Purity:>95% HPLC,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Biologically active:Yes,
Biological activity:Measured in a cell proliferation assay using murine 2E8 cells. The ED50-for this effect is-0.1 - 0.5 ng/mL.,
Accession:P13232,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.,
Storage buffer:Constituents: PBS, 8% Trehalose

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage duration-A few days, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, -80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
Biological function summary
IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.
Pathways
IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.
IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.