Abcam, ab316132, Mouse DAP12 Total ELISA Kit

Size: 1 x 96Tests
Mouse DAP12 Total ELISA Kit is a single-wash 90-min Simplestep used to detect Mouse DAP12 Total. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Mouse,
Assay type:Semi-quantitative,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse DAP12 Total in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of total mouse DAP12 protein in mouse cells.
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or controls are added to the wells, followed by the Antibody Cocktail. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C