Abcam, ab316267, Anti-KRAS 4B isoform antibody [EPR26089-79]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal RASK antibody. Suitable for WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26089-79,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Recommend to load 40-50 micrograms per lane to see a clear signal.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KRAS 4B isoform also known as K-Ras4B or simply KRAS acts as a small GTPase a type of enzyme that helps in the conversion of guanosine triphosphate (GTP) to guanosine diphosphate (GDP). KRAS 4B is approximately 21 kDa in mass. It functions by cycling between an active GTP-bound state and an inactive GDP-bound state. Expression of KRAS 4B is found mainly in the epithelial cells and it is especially abundant in the lungs colon and pancreas. Its localization is significant for its role in signaling and regulation of cellular functions.
Biological function summary
KRAS 4B isoform plays an essential role in signal transduction processes that govern cell growth differentiation and survival. It operates within complex signaling networks but does not bind with a specific complex itself. KRAS 4B is part of the Ras superfamily of proteins which are pivotal in the transduction of signals from cell surface receptors to the nucleus. Its activity influences many cellular outcomes and dysregulation can lead to aberrant cellular processes.
Pathways
KRAS 4B is critically involved in the MAPK (Mitogen-Activated Protein Kinase) and PI3K/AKT (Phosphoinositide 3-kinase/Protein Kinase B) pathways which are key in cell proliferation and survival. KRAS 4B interacts closely with other proteins such as RAF and PI3K serving as a nexus for transmitting extracellular signals to intracellular effectors. These pathways facilitate diverse biological outcomes depending on the context and cellular environment.
KRAS 4B holds a strong connection with several types of cancer particularly colorectal and pancreatic cancers. Mutations in the KRAS gene can lead to constitutive activation of the protein which promotes uncontrolled cell division and tumor growth. In these contexts KRAS 4B often interacts with other proteins like SOS (Son of Sevenless) and RAF to propagate the oncogenic signaling cascades. Therapeutic targeting of the KRAS pathway remains a challenging but vital area in cancer treatment research.