Abcam, ab31630, Anti-CD68 antibody [ED1]

Size: 125µg
Anti-CD68 antibody [ED1] (ab31630) is a mouse monoclonal antibody detecting CD68 in Flow Cytometry (Intra), Flow Cytometry . Suitable for Rat . - Over 400 publications - Trusted since 2006
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:ED1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.

Product details:
Product Specifications
Anti-CD68 antibody [ED1] (ab31630) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra) in rat samples.
Anti-CD68 antibody [ED1] (ab31630) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 125 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-CD68 antibody [ED1] (ab31630) has high sensitivity and specificity.
Anti-CD68 antibody [ED1] (ab31630) has been cited over 403 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD68 antibody [ED1] (ab31630) has 20 independent reviews from customers.
Target Information
CD68 is a transmembrane glycoprotein predominantly expressed on macrophages, playing a crucial role in phagocytosis and antigen presentation. It is involved in various pathological conditions, including neuroinflammatory diseases, where it helps clear cellular debris and modulate immune response. CD68 expression is associated with several diseases, particularly those involving macrophage activity, including, cancer, neuroinflammation, Granular cell tumor and histiocytic sarcoma.
This product should be stored undiluted.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from tissue culture supernatant., Storage buffer-pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary
CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.
Pathways
CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.
CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.