Abcam, ab316357, Anti-HLA-ABC antibody [Bu8]

Size: 100µL
Mouse Monoclonal HLA A antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Native Cell within Human HLA-A.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Bu8,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Cell within Human HLA-A.P04439

Properties and Storage Information:
Form-Liquid, Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1 month, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HLA-A HLA-C and HLA-B are molecules from the human leukocyte antigen (HLA) class I family. These proteins help present peptide antigens to T cells and function in immune surveillance. Alternate names for these targets include MHC class I where MHC stands for Major Histocompatibility Complex. The molecular mass of the HLA class I molecule is approximately 45 kDa. These proteins have expression across almost all nucleated cells. A heavy chain and a light chain β2-microglobulin form these molecules and each plays a role in antigen presentation.
Biological function summary
These proteins play an important role in immune response modulation by presenting peptide fragments on the cell surface for recognition by CD8+ cytotoxic T cells. They operate as part of the HLA class I-peptide complex which informs the immune system about the health status of cells. Healthy cells present self-peptides while infected or cancerous cells display foreign or abnormal peptides. The signaling from HLA class I to T cells can either activate or inhibit immune responses depending on the context of the peptides presented. This ensures a targeted immune response which is important for maintaining health.
Pathways
HLA-A HLA-C and HLA-B are central to the antigen processing and presentation pathway as well as the immune checkpoint pathway. These proteins interact with various components involved in these pathways such as transporter associated with antigen processing (TAP) and Protein Disulfide Isomerase (PDI) which help in peptide translocation and loading onto MHC class I molecules. The interactions within these pathways help control the immune system's ability to distinguish between self and non-self-antigens ensuring precise immune regulation.
The expression or malfunction of HLA-A HLA-C and HLA-B links tightly to autoimmune diseases and transplant rejection. Altered HLA expression can dysregulate immune responses leading to diseases like rheumatoid arthritis. Additionally variations in these antigens impact how the immune system recognizes and attacks transplanted organs potentially causing rejection. These HLA molecules also work in connection with other proteins like cytokines impacting their role and the immune response in disease contexts.