Abcam, ab316457, Recombinant Human Pro-Collagen I C-Terminal Propeptide Protein Standard (His tag)

Size: 10µg
Recombinant Human Pro-Collagen I C-Terminal Propeptide Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
Key facts
Purity:>80% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:sELISA, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P02452,
Animal free:Yes,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.3 - 7.5Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Product details:
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Procollagen Type 1 also known as Pro-1 collagen is a precursor molecule for collagen Type I which is the most abundant protein in the human body. Its molecular mass is approximately 230 kDa. This protein is synthesized by fibroblasts and is widely expressed in connective tissues including skin tendons and bones. Once synthesized procollagen Type 1 undergoes post-translational modifications and is cleaved by specific enzymes to form mature collagen fibers contributing to the structural integrity of the extracellular matrix.
Biological function summary
The synthesis and processing of procollagen Type 1 play a major role in maintaining tissue strength and elasticity. This molecule is not part of a larger complex but undergoes self-assembly to form the rigid triple-helical structure typical of collagen fibers. These fibers are essential for providing mechanical support to tissues and facilitating cell adhesion. Additionally its presence is often required for normal wound healing processes and tissue regeneration.
Pathways
The secretion and assembly of procollagen Type 1 occur through the secretory pathway. Transforming growth factor-beta (TGF-beta) signaling significantly regulates this process influencing fibrogenesis. This pathway also involves the interaction with proteins such as fibronectin and integrins which are important for aligning collagen fibers within the extracellular matrix. Furthermore procollagen Type 1 engages in the Wnt signaling pathway affecting the arrangement and stability of collagen networks.
Mutations or dysregulation of procollagen Type 1 synthesis may contribute to conditions such as osteogenesis imperfecta and scleroderma. In osteogenesis imperfecta defective collagen leads to brittle bones due to improper fiber formation. In scleroderma an overproduction of collagen causes skin thickening and organ fibrosis. The altered interaction of procollagen Type 1 with proteins like lysyl oxidase can exacerbate these pathological conditions highlighting the significance of proper collagen processing and assembly for tissue health.