Abcam, ab316489, Recombinant Human Apo-H Protein Standard (His tag)

Size: 10µg
Recombinant Human Apo-H Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
Key facts
Purity:>80% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P02749,
Animal free:Yes,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.3 - 7.5Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Product details:
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein H (Apo-H) also known as β2-glycoprotein I functions mechanically as a plasma protein. It has a molecular weight of approximately 50 kDa and it is mostly expressed in the liver. Apo-H plays a role in the body's immune and coagulation systems binding to anionic surfaces such as phospholipids and preventing blood coagulation. Its ability to interact with negatively charged molecules makes it important in the modulation of the coagulation cascade helping maintain balance between bleeding and clotting.
Biological function summary
Apo-H acts as an anticoagulant and participates in the modulation of immune response. It forms complexes with lipid-bound proteins facilitating the clearance of these complexes from the bloodstream. This interaction is key in processes like platelet aggregation and stabilization of membranes. Apo-H also influences the removal of oxidized proteins and lipids reducing cellular stress and preventing tissue damage.
Pathways
Apo-H plays a critical role in the coagulation cascade and lipid metabolism. It interacts with proteins such as prothrombin and fibrinogen influencing blood coagulation pathways. Apo-H's involvement in the clearance of apoptotic cells integrates into the lipid metabolic pathway highlighting its relationship with apolipoproteins and other lipid-related proteins. These interactions illustrate the multi-functional aspects of Apo-H within these pathways.
Apo-H associates with antiphospholipid syndrome and cardiovascular diseases. These conditions arise from its role in coagulation and immune response where disruptions can lead to increased risk of thrombosis. The protein's interaction with other apolipoproteins such as Apo-A1 highlights possible implications in lipid metabolism disorders which in turn can exacerbate cardiovascular risk. Understanding these associations provides insights into potential therapeutic targets for treating these diseases.