Abcam, ab316816, Anti-VEGFD antibody [EPR24690-17] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal VEGFD antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24690-17,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab316816 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat VEGFD. The recommended pair for sandwich ELISA is:
Capture:
ab244740
, Rat VEGFD Capture Antibody (unconjugated)
Detector: ab316816, Rat VEGFD Detector Antibody (unconjugated)
The reference range value is 3.91-1000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VEGFD also known as vascular endothelial growth factor D plays a role in angiogenesis and lymphangiogenesis by interacting with VEGF receptors. This protein has a molecular mass of approximately 32 kDa. It is typically expressed in the lungs heart skeletal muscle and small intestine. VEGFD undergoes several proteolytic processes to gain full activity highlighting its functional complexity in the body.
Biological function summary
Vascular endothelial growth factor D promotes the proliferation and migration of endothelial cells facilitating vascular formation and remodeling. It forms part of the VEGF protein family which is known for its involvement in various vascular processes. The protein's action is largely mediated through its binding to VEGFR-2 and VEGFR-3. This binding activates downstream signaling pathways that contribute to the growth and maintenance of blood and lymphatic vessels.
Pathways
The functioning of VEGFD is important in the vascular endothelial growth factor signaling pathway and the PI3K-Akt signaling pathway. These pathways are essential for the regulation of cellular processes such as survival growth and motility of endothelial cells. VEGFD is closely related to other members of the VEGF family including VEGFA and VEGFC which similarly interact with VEGF receptors to regulate vascular development and function.
Abnormal levels or activity of VEGFD can lead to pathological conditions such as cancer and lymphedema. In several cancers VEGFD is implicated in tumor angiogenesis aiding the tumor in acquiring nutrients and oxygen. Furthermore in lymphedema issues related to lymphatic vessel development can involve VEGFD alongside VEGFC contributing to fluid retention and tissue swelling. These associations highlight the importance of understanding VEGFD's functions in various pathological contexts.