Abcam, ab316931, Anti-PPP1R1A antibody [EPR28192-90]

Size: 10µL-TRIAL / 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal PPP1R1A antibody. Suitable for IP, Flow Cyt (Intra), IHC-P, ICC/IF, WB and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28192-90,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, Flow Cyt (Intra), IHC-P, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein phosphatase 1 regulatory inhibitor subunit 1A also known as PPP1R1A or inhibitor-1 (I-1) plays a significant role in cellular regulation as a phosphatase inhibitor. Mechanically it regulates the activity of protein phosphatase 1 (PP1) an essential enzyme in various cellular processes. Its molecular weight is approximately 19 kDa. PPP1R1A is expressed in many tissues showing notable levels in cardiac and skeletal muscle where it helps modulate responses to various stimuli.
Biological function summary
PPP1R1A modulates signaling pathways by influencing the dephosphorylation of substrates which is a critical step in signal transduction. It is not typically part of a complex but interacts closely with protein phosphatase 1. This interaction allows for the effective regulation of phosphorylation states within cells impacting cell cycle control muscle contraction glycogen metabolism and more. Its regulatory capabilities are important for maintaining cellular homeostasis.
Pathways
The regulatory activities of PPP1R1A significantly impact the cAMP signaling pathway and the regulation of glycogen metabolism. Within the cAMP pathway PPP1R1A regulates the activity of PP1 influencing processes like muscle contraction and gene expression. It interacts with proteins such as PKA which phosphorylates PPP1R1A activating its inhibitory function. This regulation affects downstream cellular activities by modulating phosphorylation levels of key substrates within these pathways.
Alterations in PPP1R1A expression or function have implications in cardiac diseases and diabetes. Increased or decreased activity of PPP1R1A can disrupt normal heart function due to its regulatory role in cardiac muscle contraction. Altered regulation within the cAMP and glycogen metabolism pathways can contribute to metabolic disorders like diabetes. Interactions with proteins such as PP1 and PKA are critical for these conditions highlighting PPP1R1A's role in disease progression and potential therapeutic targeting.