Abcam, ab316945, Alexa Fluor® 488 Anti-M6PR (cation dependent) antibody [EPR7691]

Size: 100µL
Rabbit Recombinant Monoclonal M6PR (cation dependent) antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7691,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle|Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
M6PR or mannose-6-phosphate receptor (cation dependent) is a protein involved in the transport of lysosomal enzymes from the Golgi apparatus to the lysosome. Alternate names for M6PR include CD-MPR and MPR46 reflecting its function and molecular weight of approximately 46 kDa. M6PR is expressed in various tissues but primarily found in the Golgi apparatus where it engages with lysosomal enzymes. This receptor is an important element in the delivery system that ensures lysosomal enzymes reach their intended cellular destination.
Biological function summary
M6PR acts as a sorting receptor by binding mannose-6-phosphate-tagged enzymes in the Golgi. It is involved in a complex trafficking mechanism that involves clathrin-coated vesicles. The receptor recognizes and binds to its ligands in the Golgi directing them to endosomes and eventually fusing with lysosomes. M6PR plays a critical role in maintaining lysosome functionality by regulating enzyme availability which is essential for cellular waste degradation and resource recycling.
Pathways
The protein M6PR participates in the lysosomal enzyme targeting pathway that ensures proper enzyme sorting and delivery within cells. This pathway overlaps with the Golgi-to-endosome transport vesicle biogenesis involving proteins like clathrin and adaptin. M6PR interacts with proteins in the sorting pathway such as GGA adaptors and Hsc70 which assist in the recognition and transport processes necessary for lysosome integrity and function.
The malfunction of M6PR links to disorders like I-cell disease and mucolipidosis II characterized by defective lysosomal enzyme targeting. Impaired M6PR function can lead to the accumulation of undigested substrates within the lysosome causing cellular dysfunction. The receptor's failure can also indirectly affect protein interactions in diseases like mucolipidosis where relationships with proteins such as IGF2R can get altered further influencing cellular pathways and leading to various pathological conditions.