Abcam, ab317042, Anti-NEFH antibody [EPR20020] - Neuronal Marker - Chicken IgY (Chimeric)

Size: 20µL / 100µL / 1mL
Chicken Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Chicken,
Clonality:Monoclonal,
Clone number:EPR20020,
Isotype:IgY,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for rat ICC. WB not recommend cell lysate.

Product details:
This Chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (
ab207176
). By design, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Thiophilic Resin, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NEFH also known as Neurofilament Heavy Chain or NEF3 is a structural protein with a molecular mass of approximately 112 kDa. This protein is primarily expressed in neurons especially within the axons. NEFH plays a mechanical role in maintaining the structural stability and caliber of axons. It is a major constituent of the axoskeleton and contributes to the maintenance of neuronal shape. NEFH acts as a scaffold allowing other proteins to assemble onto it forming neurofilament complexes that provide support to neuronal cells.
Biological function summary
Neurofilament proteins like NEFH contribute to the development and maintenance of neuron shape. NEFH exists as part of a larger neurofilament complex which includes two other major neurofilament subunits: NEFL (neurofilament light) and NEFM (neurofilament medium). Together these protein components form long filaments giving neurons their tensile strength. NEFH is important in dictating the radial growth of axons facilitating efficient nerve conduction.
Pathways
Proteins involved in axonal transport and cytoskeletal organization engage with NEFH. In the axonal transport pathway NEFH associates with proteins such as dynein and kinesin necessary for the movement of organelles along the axonal microtubules. Furthermore NEFH's role in the cytoskeletal organization pathway involves interaction with actin and microtubule-associated proteins to ensure proper neuron function and structural integrity.
Neurodegenerative conditions such as Amyotrophic Lateral Sclerosis (ALS) and some forms of Charcot-Marie-Tooth disease link NEFH involvement. Accumulation of NEFH in motor neurons has been observed in ALS indicating disruption in axonal transport. In these diseases alterations in NEFH protein interactions such as with NEFL can lead to disrupted neurofilament integrity and axonal stability contributing to neuronal dysfunction and disease progression.