Abcam, ab317100, Anti-AF9 antibody [HL1644]

Size: 100µL
Rabbit Monoclonal AF9 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MLLT3.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1644,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MLLT3.P42568

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The AF9 protein also known as AF-9 or AFNINE mechanically functions as a transcriptional coactivator involved in the regulation of gene expression. It has a molecular mass of approximately 62 kDa. AF9 expresses in various tissues including hematopoietic cells and plays an essential role in cellular transcription processes. The protein contains important domains that facilitate its interactions enabling it to modulate transcription by binding with other regulatory proteins.
Biological function summary
Functions related to AF9 involve its inclusion in the super elongation complex (SEC) which is important for transcriptional elongation. This complex controls the transition of RNA polymerase II into productive elongation stages during transcription. AF9 interacts with other proteins such as MLL ENL and P-TEFb within the SEC enhancing transcription of certain genes vital for cell proliferation and differentiation.
Pathways
AF9 plays significant roles in pathways like the MLL fusion pathway and the transcriptional regulation pathway. These pathways are integral to normal cellular processes as well as oncogenic transformations. In these pathways AF9 functionally relates to proteins such as Cyclin T1 and CDK9 through interactions within the super elongation complex modulating the transcription of genes involved in cell growth and division.
AF9 has strong links to leukemia particularly mixed lineage leukemia (MLL) due to its involvement in chromosomal translocations that create oncogenic MLL-AF9 fusion proteins. These fusions lead to altered transcription programs driving leukemogenesis. AF9 also connects to other oncoproteins like ENL that participate in similar pathways associated with MLL-related leukemias highlighting its critical role in disease pathogenesis.