Abcam, ab317135, Anti-GNAI1 + GNAI2 + GNAI3 antibody [HL2093]

Size: 100µL
Rabbit Monoclonal GNAI1 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human, Mouse, Rat, Zebrafish samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2093,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat, Zebrafish,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNAI3 GNAI1 and GNAI2 also known as Gi alpha subunits belong to the G protein family which are involved in intracellular signaling. These proteins have molecular masses of approximately 40 kDa. They are expressed in a variety of tissues including the brain heart and immune cells. Each subunit interacts with the beta and gamma subunits of G proteins to form a complex which plays a role in signal transduction by inhibiting adenylate cyclase activity leading to decreased levels of cAMP.
Biological function summary
Gi alpha subunits regulate various physiological processes such as neurotransmission and immune response. They are integral parts of receptor-mediated signaling pathways binding to G-protein-coupled receptors (GPCRs) on cell surfaces. This interaction triggers changes in cAMP levels which in turn affects cellular responses to external stimuli. Gi alpha proteins serve as molecular switches turning on or off specific signaling pathways.
Pathways
Gi alpha proteins are involved in the regulation of the cAMP signaling and calcium signaling pathways. They interact with proteins such as beta-adrenergic receptors which modulate heart rate and force of contraction. GNAI3 GNAI1 and GNAI2 relay signals from various GPCRs playing key roles in translating extracellular signals into cellular actions. Such pathways are essential for maintaining homeostasis and responding to environmental changes.
Aberrations in Gi alpha protein functions associate with conditions such as pertussis and cardiovascular diseases. Pertussis toxin targets these proteins disrupting their normal function and affecting immune response. Additionally abnormal signaling through Gi alpha-related pathways is linked to hypertension as it involves other proteins in the renin-angiotensin system. Understanding the role of these subunits aids in the development of therapeutic interventions targeting GPCR-related pathologies.