Abcam, ab317160, Anti-PFKFB4 antibody [HL2204]

Size: 100µL
Rabbit Monoclonal PFKFB4 antibody. Suitable for WB and reacts with Transfected cell lysate, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB4.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2204,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PFKFB4.Q16877

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PFKFB4 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 4 is an enzyme with a molecular mass of approximately 66 kDa. It regulates the levels of fructose-26-bisphosphate by catalyzing both its synthesis and degradation. This enzyme predominantly exists in the cytoplasm and shows higher expression in tissues with fast cell growth and high glycolytic demand like the liver and various cancerous cells. PFKFB4 serves as an important regulatory node in cellular metabolism by controlling the flux of glucose through glycolysis.
Biological function summary
PFKFB4 influences metabolic reprogramming during cell proliferation and stress conditions. It is particularly important in the glycolytic shift known as the Warburg effect where cancer cells prefer glycolysis over oxidative phosphorylation leading to rapid glycolysis and lactic acid production even in the presence of oxygen. The enzyme is often part of a complex involving other glycolytic enzymes affecting both energy production and biosynthetic processes necessary for tumor growth and survival.
Pathways
PFKFB4 plays an integral role in the glycolytic and pentose phosphate pathways. It regulates glycolysis by modulating the concentration of fructose-26-bisphosphate which is an allosteric activator of phosphofructokinase-1 (PFK-1). PFKFB4 also interfaces with AMPK signaling which ties energy status of the cell to metabolic pathways further linking it to responses to energy stress and hypoxia in cancer cells.
PFKFB4 has strong associations with cancer and metabolic disorders. Overexpression of PFKFB4 is found in several cancer types including breast and prostate cancer where it supports anabolic processes and survival under metabolic stress. The enzyme closely relates to oncogenic proteins such as MYC which drives glycolytic gene expression and HIF-1α a factor that promotes glycolysis under hypoxia. Analyzing PFKFB4 provides insights into therapeutic approaches focused on targeting metabolic pathways in cancer.