Abcam, ab317214, Anti-SARS-CoV-2 (COVID-19) nsp11 antibody [HL2388]

Size: 100µL
Rabbit Monoclonal R1A antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - SARS-CoV-2, Transfected cell line - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2388,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp11 also known as non-structural protein 11 is a small protein produced by the virus during its replication process. It is approximately 13 amino acids in length making it one of the smallest proteins encoded by the viral genome. The protein is expressed within infected host cells and is one of the multiple non-structural proteins important for the virus's replication machinery. Nsp11 is created as a part of the polyprotein processing that occurs when the viral RNA gets translated by the host cell ribosome.
Biological function summary
The function of nsp11 remains less well-characterized compared to other non-structural proteins. However it is involved in the early stages of viral RNA synthesis hinting at a role in replication or transcription regulation. Nsp11 forms part of the replication-transcription complex a multi-subunit protein assembly essential for the virus to replicate its genome inside the host cell. This complex interacts with other non-structural proteins like nsp7 nsp8 and nsp12 showing its close association within the functional viral replication system.
Pathways
Nsp11 is engaged in the virus-host interaction pathway and the viral lifecycle pathway. It interacts indirectly with host cellular processes although the precise mechanisms remain under investigation. The viral lifecycle pathway involves proteins such as nsp12 (the RNA-dependent RNA polymerase) and nsp13 (the helicase) which work together to synthesize the viral RNA demonstrating the interconnected nature of nsp11 within the larger replication machinery. It likely helps facilitate the proper assembly or function of these complexes although more specific roles need clarity.
Nsp11 is intimately connected to COVID-19 the disease caused by SARS-CoV-2 infection. As a component of the replication complex it plays a role in the virus's ability to multiply and spread within the host contributing to the disease's severity. Some studies suggest disruptions or mutations in non-structural proteins including nsp11 may alter disease outcomes although comprehensive insights are still developing. Connections to proteins like nsp12 highlight its potential impact on antiviral drug targeting as inhibiting its interaction with these proteins may reduce viral replication efficiency and therefore disease progression.