Abcam, ab317377, Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [S05-2H9]

Size: 100µL
Rabbit Monoclonal ERK1 phospho T202 antibody. Suitable for WB and reacts with Rat, Chinese hamster samples. Immunogen corresponding to Synthetic Peptide within Human MAPK1 phospho T185.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:S05-2H9,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Chinese hamster,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MAPK1 phospho T185.P28482

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERK1 and ERK2 also known as p44 and p42 MAPK respectively are important proteins in the MAP kinase signaling pathway. They are expressed in various tissues with significant presence in the brain lungs and skin. ERK1 has a molecular weight of approximately 44 kDa while ERK2 has a molecular weight of around 42 kDa. Both proteins become activated through phosphorylation which is essential for their function in cellular processes.
Biological function summary
ERK1 and ERK2 serve as key players in cellular growth differentiation and survival. They form part of a complex cascade where they transduce signals from the cell membrane to the nucleus after activation by phosphorylation. This phosphorylation enables them to modify various downstream targets involved in regulating gene expression and cellular response to external stimuli.
Pathways
ERK1 and ERK2 are critical components of the MAPK/ERK pathway and the Ras-Raf-MEK-ERK signaling cascade. These pathways regulate a multitude of cellular activities including proliferation and differentiation. In the MAPK/ERK pathway proteins like Ras and Raf serve as upstream activators of ERK1 and ERK2. Both ERK1 and ERK2 also interact with other signaling proteins such as MEK1/2 which directly phosphorylates and activates them.
Dysregulation of ERK1 and ERK2 is associated with various pathologies including cancer and neurodegenerative diseases. Abnormal activation of these proteins often leads to uncontrolled cell proliferation contributing to oncogenesis. For instance mutations in proteins like Ras which regulate ERK1 and ERK2 can result in continuous activation and lead to tumor formation. Furthermore altered ERK1 and ERK2 signaling is linked to neurodegeneration impacting neuronal survival and function.