Abcam, ab317726, Anti-PRMT3 antibody [RAB-C372]

Size: 100µg
Human Recombinant Monoclonal PRMT3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PRMT3 aa 200 to C-terminus.
Key facts
Host species:Human,
Clonality:Monoclonal,
Clone number:RAB-C372,
Isotype:Unknown,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PRMT3 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O60678,
Specificity:This antibody recognizes PRMT3 (Protein arginine N-methyltransferase 3). It binds to a folded domain, amino acids 211-531.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified by Immobilized Metal Affinity Chromatography., Storage buffer-pH: 7.2Preservative: 0.02% Proclin 300Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein arginine methyltransferase 3 (PRMT3) plays an important role in the methylation of arginine residues on various substrates which influences protein-protein interactions signal transduction and gene expression. Also known as HRMT1L3 PRMT3 has an approximate molecular weight of 59 kDa. Researchers find this enzyme mainly in the cytoplasm although it can also localize in the nucleus. Expression of PRMT3 happens across multiple tissues with notable presence in the liver and lungs.
Biological function summary
PRMT3 methylates ribosomal protein S2 an activity that suggests its participation in ribosome assembly and nucleolar functions. It forms a complex with ribosomal proteins and several metabolic enzymes which highlight its involvement in protein synthesis and cellular metabolic regulation. The activity of PRMT3 regulates the biogenesis of ribosomes which are central to protein production in cells.
Pathways
PRMT3 influences various cellular processes like the ribosome biogenesis and the mTOR signaling pathway. Its interaction with the ribosome synthesis pathway aids in the maturation and assembly of ribosomal subunits. In the mTOR pathway PRMT3 relates to S6 kinase and eukaryotic initiation factors affecting cell growth and metabolism through the regulation of protein synthesis.
PRMT3 may influence the development of metabolic syndromes and certain cancers. Studies suggest a potential link between PRMT3 dysregulation and the onset of insulin resistance making it significant in the context of type 2 diabetes. Additionally abnormal PRMT3 expression connects with progression of malignancies potentially through interacting with oncogenic proteins and altering signaling pathways that regulate cell cycle and apoptosis.