Abcam, ab317826, Anti-ECT2 antibody [EPR29014-24] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ECT2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR29014-24,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab317826 is the carrier-free version of
ab317825
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ECT2 also known as epithelial cell transforming sequence 2 is a guanine nucleotide exchange factor. It has a molecular mass of about 103 kDa. ECT2 is mostly expressed in proliferating cells including tumor cells and plays a role in cell cycle regulation. In its mechanical function ECT2 activates Rho family GTPases especially RhoA by facilitating the exchange of GDP for GTP. This activation is important for the regulation of cytoskeletal dynamics and cell division.
Biological function summary
The regulation of cytoskeletal rearrangement and cell cycle progression involves ECT2. It is an essential part of the mitotic spindle assembly complex. ECT2 interacts with several cellular components to coordinate proper cytokinesis. This activity ensures that the cells divide correctly maintaining proper cell number and tissue homeostasis. ECT2's interaction with other proteins also determines its role in controlling cell shape and movement contributing further to cell cycle events.
Pathways
ECT2 importantly participates in the RhoA signaling pathway which regulates the actin cytoskeleton. The proper function of this pathway is essential for cytokinesis and cell migration. ECT2's activity intersects with proteins like ROCK1 and ROCK2 which act downstream of RhoA to influence cell structure and integrity. In addition to RhoA signaling ECT2 is also involved in the Hippo pathway contributing to cell proliferation controls and tissue growth.
Scientists have found ECT2's involvement in cancer specifically in glioblastoma and breast cancer. Its overexpression in tumors correlates with increased cell proliferation and metastatic potential. In cancer progression ECT2 works with proteins like RhoA and YAP1 in Hippo signaling to influence cell growth and survival making it a target of interest in cancer research and therapeutic development. The aberrant regulation of ECT2 and its pathway partners can lead to uncontrolled cell division and tumor growth.