Abcam, ab318250, Recombinant Human Acid phosphatase/ACP1 Protein (Isoform 2) (His tag)

Size: 10µg / 50µg / 100µg
Recombinant Human Acid phosphatase/ACP1 Protein (Isoform 2) (His tag) is a Human Full Length protein, in the 1 to 158 aa range, expressed in Escherichia coli, with >95%, <0.005 EU/µg endotoxin level, suitable for Mass Spec, HPLC, SDS-PAGE.
Key facts
Purity:>95% HPLC,
Endotoxin level:<0.005 EU/µg,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass Spec, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Mass Spectrometry:LC-MS/MS,
Accession:P24666,
Animal free:Yes,
Carrier free:No,
Species:Human,
Storage buffer:pH: 6Constituents: 10% Glycerol (glycerin, glycerine), 0.2789% MES, 0.0579% EDTA, 0.0022% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
Biological function summary
The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.
Pathways
The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.
Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.