Abcam, ab318980, Anti-TIGAR antibody [EPR29002-30]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal TIGAR antibody. Suitable for WB, ICC/IF and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR29002-30,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TIGAR also known as TP53-Induced Glycolysis and Apoptosis Regulator is a protein involved in cellular metabolism. With a mass of approximately 28 kDa TIGAR regulates glycolysis by acting similarly to fructose-26-bisphosphatase. It is expressed predominantly in tissues with active cellular proliferation such as the brain and liver. TIGAR functions mechanically to inhibit the glycolytic flux by decreasing levels of fructose-26-bisphosphate which is important for various cellular activities.
Biological function summary
TIGAR plays a critical role in controlling the oxidative stress within cells. By lowering glycolytic rates it shifts cellular metabolism toward the pentose phosphate pathway boosting the production of NADPH and reducing reactive oxygen species (ROS). This protein does not form a part of a larger complex operating independently to exhibit its effects. It also influences cellular survival mechanisms by mitigating oxidative stress which is important for cell health.
Pathways
TIGAR is involved with both apoptosis and carbohydrate metabolism pathways where it acts to support cellular metabolism and survival. Within these pathways it interacts closely with the tumor suppressor protein p53 which regulates TIGAR expression. Another related protein is AMPK which also plays roles in energy balance and is influenced by changes in cellular metabolic status. TIGAR's actions help in cellular adaptation during metabolic stress maintaining energy homeostasis.
TIGAR is linked to cancer and neurodegenerative diseases. Its role in reducing oxidative stress makes it significant in cancer as elevated levels can sometimes promote cancer cell survival. Involvement with p53 is particularly notable as alterations in p53 pathways are common in various cancers. In neurodegenerative diseases such as Alzheimer's TIGAR's function in managing oxidative stress can affect neuronal survival. Dysregulation of related metabolic processes can contribute to disease progression highlighting the importance of TIGAR in these contexts.