Abcam, ab319059, Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172]

Size: 20µL / 100µL / 1mL
Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] is a Rabbit Recombinant Multiclonal antibody that is used in HDAC1 + HDAC2 + HDAC3 + HDAC8 Flow Cytometry (Intra), ICC/IF, IHC-P, IP, Western Blot. Suitable for Human, Mouse, Rat samples. Histone deacetylases 1, 2, 3, and 8 (HDAC1, HDAC2, HDAC3, HDAC8) are critical enzymes involved in chromatin remodeling and gene expression regulation. Dysfunctions in HDACs have been linked to cancer and neurodegenerative diseases such as Alzheimer's Disease.
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:RM1172,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-P, Flow Cyt (Intra), ICC/IF, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for mouse and rat IP application.

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Histone deacetylases 1 2 3 and 8 (HDAC1 HDAC2 HDAC3 HDAC8) are critical enzymes involved in chromatin remodeling and gene expression regulation. They operate by removing acetyl groups from lysine residues on histones leading to chromatin condensation and transcriptional repression. HDAC1 has a molecular weight of approximately 61 kDa HDAC2 is around 55 kDa HDAC3 approximately 48 kDa and HDAC8 about 41 kDa. These proteins are expressed in various tissues with high levels found in the brain liver and heart indicating their importance in multiple physiological processes.
Biological function summary
HDAC1 HDAC2 HDAC3 and HDAC8 play roles in transcriptional repression and cell cycle progression. They often exist as part of larger multiprotein complexes like the Sin3 NCoR and CoREST complexes which enhance their deacetylase activity. These enzymes participate in the regulation of genes involved in cell differentiation proliferation and apoptosis. Their activity is important for maintaining the balance of gene expression required for normal cellular function and development.
Pathways
HDAC1 HDAC2 HDAC3 and HDAC8 integrate into the regulation of critical signaling cascades such as the Notch and Wnt pathways. In the Notch pathway they interact with transcriptional repressors to adjust gene expression patterns during cell differentiation. Within these pathways HDAC proteins interact with other key proteins like JAG1 in the Notch pathway and β-catenin in the Wnt pathway modulating the transcriptional activity based on cellular contexts and stimuli.
Dysfunction in HDAC1 HDAC2 HDAC3 and HDAC8 links to conditions such as cancer and neurodegenerative diseases. In cancer these HDACs can promote oncogenic processes by silencing tumor suppressor genes making them targets for cancer therapy. In neurodegenerative diseases like Alzheimer's alterations in their activity may disrupt normal neuronal function and gene expression patterns. The involvement with proteins such as p53 in cancer and tau proteins in neurodegenerative disorders highlights the critical role these HDACs play in disease mechanisms.