Abcam, ab320051, HIV1 p24 ELISA Kit- Extracellular

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
HIV1 p24 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify HIV1 p24 with a sensitivity of 4.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant,
Reacts with:Human immunodeficiency virus,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.3 pg/mL,
Range:15.625 - 1000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
HIV1 p24 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HIV1 p24 protein in Serum, Cit plasma, EDTA Plasma, Hep Plasma, and Cell culture supernatant. Quantitate  HIV1 p24 with 4.3 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIV1 p24 also known as HIV p24 protein is a core protein of the Human Immunodeficiency Virus type 1 (HIV-1). The p24 protein has a molecular weight of about 24 kDa. HIV1 p24 comprises part of the viral capsid which encases the viral RNA genome. This protein is prominently expressed during the early stages of HIV infection. The presence of HIV1 p24 is commonly detected through various laboratory techniques including the use of p24 ELISA kits.
Biological function summary
HIV1 p24 plays an important role in the viral life cycle. It assists in the formation and stability of the viral capsid which is critical for maintaining the integrity of the viral core. HIV p24 is not a lone actor; it is part of the structural framework and interacts with other viral proteins to facilitate the viral assembly and maturation processes. The detection of HIV1 p24 is a marker for viral replication and infection stage.
Pathways
The function of HIV1 p24 aligns significantly within the HIV replication pathway and the host’s immune response pathway. HIV p24 is intrinsically tied to processes of viral assembly alongside proteins like Gag and Pol coordinating to ensure successful viral replication. Furthermore the immune system recognizes the HIV1 p24 protein thereby integrating it into the host immune response which is important in disease progression and for diagnostic purposes like in the HIV ELISA assay.
HIV1 p24 is integrally connected to HIV/AIDS a disorder that profoundly affects the immune system. The presence of HIV p24 in the bloodstream is an early marker of infection and is frequently monitored to track the disease's progression. Alterations in the levels of p24 protein relate to the efficiency of therapeutic interventions. The study of HIV1 p24 also brings focus on its interaction with the CD4 receptor which is pivotal in the viral entry process further linking various stages of the HIV infection cycle to potential therapeutic targets.