Abcam, ab320090, Anti-SARS-CoV2 nsp4 antibody [EPR24843-87]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - SARS-CoV-2, Recombinant full length protein - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24843-87,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:WB, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV2 nsp4 or non-structural protein 4 is a component of the viral replication and transcription complex. Nsp4 is about 54 kilodaltons in mass and is produced by the cleavage of polyprotein 1a/1ab. This protein mainly expresses in host cells where the virus replicates. During infection nsp4 partners with other non-structural proteins to modulate the microenvironment needed for viral RNA synthesis forming part of the membrane-associated complex important for coronavirus replication.
Biological function summary
Nsp4 along with nsp3 and nsp6 plays an important role in remodeling host cell membranes to form double-membrane vesicles (DMVs) which serve as sites for viral replication. These vesicles protect viral RNA from host cell defenses. Nsp4 contributes to membrane rearrangements that create optimal conditions for viral replication complexes. By cooperating within this multi-protein machinery nsp4 facilitates efficient RNA synthesis and assembly of new virions.
Pathways
Interactions involving nsp4 form important links in the viral life cycle. One pivotal pathway is the formation of DMVs which is essential for viral RNA replication and protects it from degradation. The viral replication machinery also involves nsp3 and nsp6 allowing it to effectively exploit host cell machinery. Further nsp4 interacts with key components of the endoplasmic reticulum manipulating cellular processes for the benefit of the virus.
Nsp4 holds significant implications in COVID-19. Infection with SARS-CoV-2 leads to respiratory illness with varying degrees of severity. Nsp4 by participating in forming replication vesicles aids viral propagation contributing to viral load and disease progression. The virus's prolific replication mediated by proteins like nsp4 can overwhelm the immune response and is linked with severe outcomes. Interactions between nsp4 and other non-structural proteins like nsp3 suggest potential targets for antiviral therapies aiming to dismantle the replication complex.