Abcam, ab32072, Anti-c-Myc antibody [Y69] - ChIP Grade

Size: 20µL / 100µL / 1mL
Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) is a rabbit monoclonal antibody detecting c-Myc in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 1320 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y69,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, IP, ChIC/CUT&RUN-seq, ChIP-seq, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for endogenous c-Myc. It does not detect Myc tag. Expression levels of the target protein vary with sample type and some optimization may be required.Please see documentation for further information on positive controls (Chinese version)

Product details:
Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP,-seq Flow Cyt (Intra), ICC/IF, IHC-P and IP.
Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) was first used in a scientific publication in 1985 and has been cited over 1320 times in peer reviewed journals. It's performance in Western blot and IHC in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has been confirmed by Western Blot testing in c-Myc knockout HEK-293T cells (
ab256500
Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has 55 independent reviews from customers.
c-Myc antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has been validated in
ab256500
ChIC/CUT&RUN-seq, ChIP,-seq Flow Cyt (Intra), ICC/IF, IHC-P and IP.
Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) specifically detects c-Myc (UniProt ID: P01106; Molecular weight: 51kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone Y69 -
ab168727
Antibody clone Y69 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
594, Alexa Fluor
555, HRP, FITC (
ab190026
ab190560
ab201775
ab201780
ab205818
ab223913
Only antibody in the market to this target validated for both CUT&RUN and ChIP-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS. Highly-specific antibody targeting c-Myc, a crucial transcription factor involved in various cellular processes including cancer biology, cell cycle regulation and apoptosis studies. Widely used in cancer research, this antibody is crucial for investigating c-Myc's role in tumorigenesis, including Burkitt lymphoma, multiple myeloma and glioblastoma.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The c-Myc protein also known as Myc or cMyc is a transcription factor with a molecular weight of approximately 49 kDa. It plays a mechanical role by binding to DNA where it regulates the expression of various genes. Researchers often detect c-Myc in the cell nucleus. It expresses in many cell types including embryonic stem cells and cancer cells. Due to its influential role in proliferation and growth scientists have highly studied c-Myc for its functions and implications.
Biological function summary
C-Myc influences cellular processes like cell cycle progression apoptosis and metabolism. It often forms a complex with its partner protein Max to bind target genes and activate transcription. This c-Myc/Max complex operates by manipulating gene expression patterns which leads to changes in cellular behavior. Through its activity c-Myc affects cell growth and division which remain critical for development and homeostasis.
Pathways
Many scientists understand that c-Myc serves major functions in the MYC signaling pathway and the PI3K/AKT signaling pathway. c-Myc modulates the expression of genes that govern cell cycle checkpoints and cellular metabolism through these pathways. In this context c-Myc interacts with proteins like TCF and cyclins allowing the fine-tuning of cell proliferation and growth signals. These pathway involvements highlight the protein's importance in fundamental cellular mechanisms and its regulation.
Researchers have frequently associated c-Myc with cancer and cardiovascular disorders. In cancer c-Myc overexpression can drive tumorigenesis by promoting uncontrolled cell proliferation. This often involves interactions with other oncogenic proteins like Bcl-2. In cardiovascular disorders c-Myc dysregulation can disrupt normal cellular function and contribute to pathophysiological conditions. Studies continue to explore c-Myc's role in these diseases aiming to develop therapeutic strategies targeting its activity.