Abcam, ab320741, Anti-SPHK2 antibody [EPR29189-33]

Size: 20µL / 100µL / 1mL
Knockout Tested Rabbit Recombinant Monoclonal SPHK2 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Rat, Transfected cell line - Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR29189-33,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WB, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPHK2 also known as sphingosine kinase 2 is an enzyme responsible for the conversion of sphingosine to sphingosine-1-phosphate (S1P). This enzyme weighs approximately 68 kDa and is expressed in a wide range of tissues including liver kidney and brain. The catalytic activity of SPHK2 involves the phosphorylation of sphingosine which plays a part in the modulation of various cellular processes.
Biological function summary
SPHK2 influences cell growth survival and differentiation by generating sphingosine-1-phosphate a signaling lipid. It functions independently or in association with other proteins but it is not always a part of a larger complex. The generated S1P acts as a signaling molecule both inside and outside of cells affecting processes like apoptosis and inflammation.
Pathways
SPHK2 is a significant player in the sphingolipid signaling pathway and impacts the MAPK/ERK pathway. These pathways collectively regulate cellular proliferation and apoptosis. SPHK2 works in conjunction with SPHK1 a closely related sphingosine kinase in these signaling routes. These kinases together help to balance the levels of sphingosine and S1P which is essential for maintaining cellular homeostasis.
SPHK2 has been linked to cancer and inflammatory diseases. The overexpression of SPHK2 can result in increased levels of sphingosine-1-phosphate promoting cancer cell survival and resistance to chemotherapy. In addition SPHK2's involvement in inflammation suggests roles in conditions like rheumatoid arthritis where it might interact with TNF-α related signaling pathways. These connections highlight SPHK2 as a potential target for therapeutic intervention in such diseases.