Abcam, ab320782, Alexa Fluor® 647 Anti-PHC2 antibody [EPR27147-176] - ChIP Grade

Size: 100µL
Rabbit Recombinant Monoclonal PHC2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27147-176,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for FC-intra human.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle|Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHC2 also known as Polyhomeotic Homolog 2 is a protein with a molecular mass around 100 kDa. It localizes mainly in the nucleus and expresses widely across different tissue types. The protein is a part of the Polycomb group (PcG) proteins which play key roles in chromatin remodeling and gene silencing. PHC2 contributes to forming stable gene repression essential for regulating development and maintaining cellular identity.
Biological function summary
PHC2 functions as a part of the Polycomb repressive complex 1 (PRC1). This complex is involved in modifying the structure of chromatin which helps maintain the repression of gene transcription. Through interaction with other PRC1 components PHC2 helps establish a compact chromatin state promoting histone modifications that lead to transcriptional silencing. This activity is fundamental for cellular processes like embryonic development and stem cell maintenance.
Pathways
PHC2 plays a role in the regulation of the Wnt signaling and Notch signaling pathways. These pathways are integral to controlling cell proliferation differentiation and apoptosis. Within these pathways PHC2 interacts with other proteins like BMI1 and RING1B which are also part of the PRC1 complex. These interactions ensure the precise control over gene expression needed for cellular homeostasis and proper developmental progression.
Aberrant PHC2 function is implicated in a range of conditions particularly cancer and neurodevelopmental disorders. Dysregulation of PHC2 can lead to misexpression of genes that regulate cell cycle and apoptosis contributing to oncogenesis. For instance PHC2 has connections with BMI1 in cancer pathways influencing tumor progression and resistance to apoptosis. Moreover mutations or irregular expression of PHC2 have been linked with neurodevelopmental disorders highlighting its importance in nervous system development and function.