Abcam, ab32157, Anti-EIF2S1 (phospho S51) antibody [E90]

Size: 20µL / 100µL / 1mL
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) is a rabbit monoclonal antibody detecting EIF2S1 in Western Blot, IHC-P, Dot Blot . Suitable for Human, Mouse, Neurospora crassa, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 270 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E90,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Neurospora crassa,
Applications:IHC-P, Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot Blot, IHC-P and WB.
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) was first used in a scientific publication in 1987 and has been cited over 274 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) has 24 independent reviews from customers.
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) specifically detects EIF2S1 Phospho-S51 (UniProt ID: P05198; Molecular weight: 37kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone E90 -
ab214434
Highly-cited antibody with >350 citations. Highly specific phospho S51 EIF2S1 antibody, essential for researchers studying eukaryotic initiation factor 2 subunit 1 and protein synthesis. This antibody is crucial in stress response research, particularly in understanding translation regulation and EIF2S1 phosphorylation. It is widely used in studies of cellular stress and EIF2S1 inhibitors
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF2S1 also called eIF2α is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
Biological function summary
EIF2α acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2α leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2α forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.
Pathways
EIF2α is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2α is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2α plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.
EIF2α connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2α are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2α signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2α in these diseases highlighting eIF2α's involvement in critical pathological processes.