Abcam, ab321891, Anti-SHARPIN antibody [EPR29120-74]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal SHARPIN antibody. Suitable for WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR29120-74,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for WB in human tissues.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHARPIN also known as SHANK-associated RH domain-interacting protein is a multi-functional protein with a molecular mass of approximately 34 kDa. It primarily acts as a ubiquitin-binding protein and is a part of the LUBAC (Linear Ubiquitin Chain Assembly Complex) involved in various cellular signaling pathways. SHARPIN is expressed in a range of tissues but is highly abundant in lymphoid tissues and the brain. Its mechanical actions involve binding and modulating ubiquitin chains which plays a role in regulating protein stability and signaling processes in cells.
Biological function summary
SHARPIN engages in significant cellular functions by being a critical component of the LUBAC complex. This complex is central to the activation of NF-kB signaling by mediating linear ubiquitination of specific substrate proteins influencing inflammatory responses and cell death regulation. In the immune system SHARPIN regulates the TNF receptor signaling and helps maintain homeostasis. Its absence or mutation can lead to dysregulated cell survival and immune responses highlighting its importance in maintaining cellular functions.
Pathways
SHARPIN integrates into the NF-kB and Wnt signaling pathways which are vital for regulating immune responses and cell fate decisions. Within the NF-kB pathway SHARPIN cooperates with other LUBAC components such as HOIP and HOIL-1L to facilitate ubiquitination necessary for pathway activation. Additionally SHARPIN influences the Wnt pathway through interactions with Dishevelled proteins contributing to developmental and stem cell signaling. The coordination of these pathways by SHARPIN highlights its role in cellular communication and signaling.
SHARPIN has connections to conditions such as chronic inflammation and immunodeficiency syndromes. Dysregulation in SHARPIN function can contribute to chronic inflammatory diseases including psoriasis due to improper NF-kB pathway regulation. Its association with HOIP and HOIL-1L through the LUBAC complex also links it to autoinflammatory conditions characterized by immune system imbalances. SHARPIN mutations are implicated in skin and immune disorders reflecting its broad impact on health and disease manifestation.