Abcam, ab322055, Anti-ATP6V1B2 antibody [EPR28916-13]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ATP6V1B2 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28916-13,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:Dot, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for mouse TMA.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V1B2 also known as V-type proton ATPase subunit B2 is an essential component of the vacuolar ATPase (V-ATPase) complex. It weighs approximately 56 kDa. This protein is responsible for the hydrolysis of ATP supplying energy for proton translocation across membranes. ATP6V1B2 is heavily expressed in various tissues including the brain and kidney. As part of the V1 domain of the V-ATPase it plays an important role in regulating intracellular pH.
Biological function summary
ATP6V1B2 contributes to several key processes by enabling proton transport critical for organelle acidification. It is a component of the V-ATPase complex a multi-subunit complex important for the acidification of intracellular compartments. Organelle acidification affects processes like protein degradation neurotransmitter loading and receptor-mediated endocytosis. ATP6V1B2 specifically aids in the stabilization and assembly of the V-ATPase complex ensuring effective proton pumping activity in diverse cellular environments.
Pathways
ATP6V1B2 plays a significant role in both endocytic and autophagic pathways. The endocytic pathway integrates proteins such as clathrin and dynamin which are important for the internalization and recycling of membrane components. In autophagy ATP6V1B2 assists in the fusion of autophagosomes with lysosomes influencing cellular homeostasis and response to nutrient deprivation. These pathways are integral for maintaining cellular energy balance growth and survival.
ATP6V1B2 has associations with conditions such as dominant and recessive cutis laxa type II and Zimmermann-Laband syndrome. These diseases often display craniofacial abnormalities and variable connective tissue symptoms. Connections with proteins like ATP6V1A have been identified which also participate in similar disorders due to shared structural roles in maintaining proper V-ATPase complex function. Understanding these links can further research into targeted therapeutic strategies.