Abcam, ab322230, Alexa Fluor® 647 Anti-EMR1/ADGRE1 antibody [EPR23225-94]

Size: 100µL
Rabbit Recombinant Monoclonal F4/80 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23225-94,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The EMR1/ADGRE1 (F4/80) is a protein known for its role in cellular adhesion and migration. It is also referred to as EMR1 or ADGRE1 and is a member of the class B seven-span transmembrane receptor family. The protein has a molecular mass of about 90 kDa and primarily expresses on the surface of tissue-resident macrophages. Its expression serves as a marker to identify murine macrophages thereby providing a way to study subsets of immune cells within diverse tissues.
Biological function summary
This protein influences the regulation of immune responses and macrophage actions. It does not appear to work alone; it forms part of a larger receptor complex that participates in interaction with other cellular components modulating biological processes such as phagocytosis and the release of cytokines. These roles position the ADGRE1 macrophage in the immune surveillance of tissues influencing homeostasis and response to injury or infection.
Pathways
ADGRE1/EMR1 (F4/80) takes part in signal transduction pathways related to the innate immune response. It interacts with proteins involved in macrophage activation and cellular signaling such as TLR4 and CD14. These pathways are instrumental in mediating responses to pathogens and tissue damage integrating the ADGRE1 marker in processes that initiate and propagate immune signaling cascades.
ADGRE1/EMR1 (F4/80) shows involvement in inflammatory conditions and autoimmune disorders. For example it is relevant to research in diseases like inflammatory bowel disease and rheumatoid arthritis where macrophage-mediated inflammation plays a significant role. In these conditions the protein interacts with cytokines like TNF-alpha linking its expression to clinical manifestations and therapeutic targets. This association makes this protein a potential focal point for understanding and potentially mitigating disease pathology.