Abcam, ab323247, Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free

Size: 100µg / 1mg
Chicken Recombinant Monoclonal Vimentin antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse samples.
Key facts
Host species:Chicken,
Clonality:Monoclonal,
Clone number:EPR3776,
Isotype:IgY,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (
ab92547
). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
ab323247 is the carrier-free version of
ab323238
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Thiophilic Resin, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pathways
Vimentin plays a role in the epithelial-to-mesenchymal transition (EMT) and interacts within the TGF-beta signaling pathway. It works alongside proteins like N-cadherin and fibronectin in the EMT pathway contributing to increased cell motility and survival. This involvement positions vimentin as a control point reflecting changes in cell phenotypes under physiological and pathological conditions.
Vimentin also known by its scientific name VIM is a type of intermediate filament protein that forms part of the cytoskeleton. It has a molecular mass of approximately 57 kDa. Vimentin is expressed in various cell types including mesenchymal cells fibroblasts and endothelial cells. This protein maintains cell integrity and provides resistance against stress by organizing cellular architecture and anchoring organelles. Researchers often use antibodies such as anti-vimentin or anti-mouse vimentin to study its expression and functions in cell biology.
Biological function summary
The role of vimentin involves providing structural support and participating in processes like cell division and wound healing. Vimentin forms complexes with other cytoskeletal proteins significantly impacting cell shape and mobility. Its involvement in cellular stability becomes paramount during events requiring cellular reorganization such as embryogenesis and inflammation. These functions are frequently observed using labeled antibodies like Alexa Fluor 568 and Alexa Fluor 647 for fluorescent imaging.
Vimentin has relevance in cancer metastasis and cardiovascular diseases. Its aberrant expression correlates with tumor progression and metastatic potential by affecting cell movement and invasion. Vimentin also associates with proteins like collagen and integrins in the context of cardiovascular pathologies influencing vascular stiffness and remodelling. By targeting vimentin researchers aim to understand intervention strategies that may mitigate these diseases.