Abcam, ab323262, Alexa Fluor® 488 Anti-Puromycin antibody [EPR27218-173]

Size: 100µL
Rabbit Recombinant Monoclonal Puromycin antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Chemical samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27218-173,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Applications:Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Puromycin is a well-known antibiotic that acts by disrupting protein synthesis. It mimics the structure of an aminoacyl-tRNA leading to premature termination of the growing polypeptide chain during translation. Puromycin is small with a molecular mass of approximately 471 daltons. It interacts mainly within the cytoplasm where it can incorporate into the peptide chain due to its similarity to the 3' end of the aminoacyl-tRNA. Puromycin does not have a specific expression site like endogenous proteins because it is not naturally occurring in animals; rather it is used experimentally.
Biological function summary
The function of puromycin is to interfere with protein synthesis by targeting ribosomes. It binds to the ribosomal A site leading to the release of incomplete polypeptides. Puromycin is not part of a complex but establishes a strong interaction with ribosomes found in prokaryotic and eukaryotic cells. This interaction gives it unique utility in experiments that require inhibition of translation such as studies involving protein turnover and synthesis rates.
Pathways
The action of puromycin strongly impacts protein synthesis pathways. It inhibits the elongation stage of the translation pathway by mimicking an aminoacyl-tRNA. Puromycin does not involve complex pathways itself but its effects can influence other proteins involved in translation such as elongation factors and the ribosomal subunits. This makes puromycin useful in basic scientific research to study how cells control protein synthesis.
Puromycin's ability to inhibit translation links it to studies on cancer and neurodegenerative diseases. Researchers use puromycin to explore how disrupting protein synthesis can affect cancer cell growth. It serves as a model for understanding toxins that affect similar pathways in neurodegenerative disorders. Through these studies connections have been made between puromycin's action and proteins involved in these diseases such as oncogenes and neuromodulatory proteins which are key to understanding disease progression and developing potential treatments.