Abcam, ab323444, Alexa Fluor® 647 Anti-MTF2 antibody [EPR27196-167]

Size: 100µL
Rabbit Recombinant Monoclonal MTF2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27196-167,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for human IHC and ICC.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle|Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MTF2 also known as metal response element binding transcription factor 2 or M96 is a component of the Polycomb group (PcG) proteins. It's a protein involved in the regulation of gene expression via chromatin remodeling. MTF2 has a mass of approximately 110 kDa. It is expressed in various tissues with significant presence in the brain and embryonic stem cells where regulatory control of gene expression plays an important role in development.
Biological function summary
MTF2 acts mainly as a recruiter of Polycomb Repressive Complex (PRC) 2 to specific gene loci. This recruitment is essential for the methylation of histone H3 at lysine 27 (H3K27me3) a modification linked to transcriptional repression. MTF2’s role in gene silencing involves interaction with multiple other PcG proteins like EZH2 a component of PRC2 which further emphasizes its central position in maintaining the transcriptional repression needed for proper cell differentiation and development.
Pathways
MTF2 plays an important part in the regulation of the Wnt and Hedgehog signaling pathways. These pathways are significant in embryonic development and cell fate decisions. MTF2 interfaces with proteins such as SUZ12 within PRC2 which modulate repression activities by influencing chromatin structure in these pathways. Through these interactions MTF2 helps manage expression levels of downstream genes ultimately affecting cellular differentiation processes.
MTF2 has been implicated in several conditions related to dysregulation of gene expression. For example aberrant function or expression of MTF2 can contribute to cancers due in part to its role in controlling cell proliferation through gene silencing. Additionally mutations or dysfunctions in MTF2 have been connected to neurological disorders because of its high expression in brain tissues. The relationship between MTF2 and proteins in the PRC2 complex such as EZH2 is of particular interest in research related to these diseases as they collectively contribute to the maintenance of gene expression patterns critical to both normal development and disease states.