Abcam, ab323445, Alexa Fluor® 488 Anti-Lamin C antibody [EPR28295-16]

Size: 100µL
Rabbit Recombinant Monoclonal Lamin-A/C antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28295-16,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin C also known as LMNA forms part of the nuclear lamina which provides structural support to the cell nucleus. It has a molecular weight of approximately 65 kDa. Lamin C is expressed in various cell types especially in differentiated cells within tissues such as muscle cardiac and certain epithelial cells. It helps to maintain nuclear stability and influences gene expression by interacting with chromatin.
Biological function summary
Lamin C contributes to the architecture of the nuclear envelope as part of the lamina complex. This structural role influences the mechanical properties of cells which affects how cells respond to mechanical stress. Lamin C associates with proteins from the inner nuclear membrane and chromatin playing an essential role in nuclear organization. Disruptions in Lamin C function can affect the physical properties of nuclei impacting gene regulation and cell function.
Pathways
Multiple processes integrate Lamin C including the mitogen-activated protein kinase (MAPK) signaling pathway and the PI3K/AKT pathway. In these pathways Lamin C can interact with proteins like MAPK and Akt which are important for transmitting signals that regulate cell growth survival and differentiation. In these processes Lamin C ensures proper cell cycle progression and nuclear structural integrity highlighting its significant involvement in cellular dynamics.
Mutations and malfunctions in Lamin C are connected to muscular dystrophies and cardiomyopathies. These diseases highlight the importance of Lamin C in maintaining muscle tissue integrity and proper cardiac function. Lamin C mutations often co-occur with dysfunctions in Lamin A as both arise from the same precursor protein leading to overlapping symptoms in conditions like Emery-Dreifuss muscular dystrophy and dilated cardiomyopathy.