Abcam, ab323699, Human C3a anaphylatoxin ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human C3a anaphylatoxin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C3a anaphylatoxin with a sensitivity of 1.745 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.745 pg/mL,
Range:3.906 - 250 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human C3a anaphylatoxin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C3a anaphylatoxin protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant. Quantitate Human C3a anaphylatoxin with 1.745 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C