Abcam, ab32376, Anti-PKC alpha antibody [Y124]

Size: 100µL / 1mL
Anti-PKC alpha antibody [Y124] (ab32376) is a rabbit monoclonal antibody detecting PKC alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Pig, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 110 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y124,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Pig,
Applications:IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-PKC alpha antibody [Y124] (ab32376) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Pig, Rat samples.
What is the molecular weight of PKC alpha?
Anti-PKC alpha [Y124] (ab32376) specifically detects a band for PKC alpha (UniProt: P17252) at a molecular weight of 77kDa.
Trusted by the scientific community
Anti-PKC alpha [Y124] (ab32376) was first used in a scientific publication in 2006 and has been cited over 110 times in peer-reviewed journals.
Reviewed by scientists
Anti-PKC alpha [Y124] (ab32376) has over 10 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-PKC alpha antibody [Y124] (ab32376) has been confirmed by Western blot testing in PRKCA Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [Y124] also available for your convenience: ab32376, Alexa Fluor® 488 -
ab204633
, Alexa Fluor® 647 -
ab204634
, HRP -
ab205422
, Alexa Fluor® 594 -
ab206344
, Alexa Fluor® 405 -
ab206345
, PE -
ab208749
, Carrier free -
ab221611
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PKC alpha known as Protein Kinase C alpha is a serine/threonine-specific protein kinase involved in various cellular processes. The molecular mass of PKC alpha is approximately 80 kDa. Alternately called PKCA or PRKC alpha it is expressed in many tissues with high expression in the brain heart and lungs. PKC alpha plays a role in signal transduction by phosphorylating serine and threonine residues on target proteins.
Biological function summary
PKC alpha regulates cellular functions including proliferation differentiation and apoptosis. It often acts as part of a larger protein complex modulating various biological responses by interacting with substrates and co-factors. Its activity is dependent on calcium ions and diacylglycerol emphasizing its role in calcium and lipid signaling pathways.
Pathways
PKC alpha plays a vital role in the MAPK/ERK pathway and the PI3K/AKT pathway which are critical for the cellular stress response and growth regulation. PKC alpha interacts with several proteins in these pathways including RAF1 and AKT1 facilitating signal transmission that influences cell fate decisions.
PKC alpha has been implicated in cancer and cardiac hypertrophy. In cancers aberrant PKC alpha signaling is linked to increased cell proliferation and survival. In cardiac hypertrophy it is associated with abnormal heart growth due to impaired signaling involving AKT1 and GSK3B. These connections highlight PKC alpha's influence in pathological as well as physiological conditions.