Abcam, ab32391, Anti-GSK3 beta antibody [Y174]

Size: 20µL / 100µL / 1mL
Anti-GSK3 beta antibody [Y174] (ab32391) is a rabbit monoclonal antibody detecting GSK3 beta in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, ELISA . Suitable for Human, . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 200 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y174,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, Flow Cyt (Intra), WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for human GSK3 beta. It may also detect the splice isoform 2 based on sequence homology.The immunogen used for this antibody is GSK3 beta phospho S9. This antibody shows partially phospho specificity to phospho S9 under certain conditions, for example, under low peptide concentration in ELISA assay, it has dominant reactivity with phospho S9 peptide.

Product details:
What is this antibody validated in?
Anti-GSK3 beta antibody [Y174] (ab32391) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, samples.
What is the molecular weight of GSK3 beta?
Anti-GSK3 beta [Y174] (ab32391) specifically detects a band for GSK3 beta (UniProt: P49841) at a molecular weight of 46kDa.
Trusted by the scientific community
Anti-GSK3 beta [Y174] (ab32391) was first used in a scientific publication in 2006 and has been cited over 200 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-GSK3 beta antibody [Y174] (ab32391) has been confirmed by Western blot testing in GSK3B Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [Y174] also available for your convenience: ab32391, Carrier free -
ab183177
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ab196910
, HRP -
ab196911
, Alexa Fluor® 488 -
ab197236
, Alexa Fluor® 594 -
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, Alexa Fluor® 555 -
ab201738
, PE -
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycogen synthase kinase 3 beta or GSK3 beta is a serine/threonine protein kinase that regulates various cellular processes. It is also referred to as GSK3B and has a molecular weight of approximately 47 kilodaltons (kDa). This enzyme is widely expressed in many tissues with notably high expression levels in the brain. Mechanically GSK3 beta phosphorylates target proteins affecting their activity stability and interactions with other proteins.
Biological function summary
As an important regulatory protein GSK3 beta plays a role in multiple cellular functions including metabolism cell cycle and cell signaling. It often interacts with components in signaling complexes and modulates the activity of these complexes involving phosphorylation. It is involved in the regulation of transcription factors such as c-Myc and Β-catenin influencing cell fate and survival. This ability to modulate diverse functions highlights GSK3 beta's importance in cellular regulation.
Pathways
GSK3 beta participates in significant signaling pathways such as the Wnt/Β-catenin and insulin signaling pathways. In the Wnt pathway GSK3 beta phosphorylates Β-catenin marking it for degradation which regulates gene transcription. In insulin signaling it interacts with proteins like AKT to mediate glycogen synthesis. These interactions highlight its role in maintaining cellular homeostasis and energy balance.
GSK3 beta has implications in Alzheimer's disease and type 2 diabetes. It is associated with tau protein hyperphosphorylation and neurofibrillary tangles in Alzheimer's disease. In diabetes its role in insulin signaling impacts glucose metabolism. Further GSK3 beta's dysfunction relates to disrupted signaling related to these conditions positioning it as a potential therapeutic target.