Abcam, ab32417, Anti-GTPase HRAS antibody [Y132]

Size: 100µL / 1mL
Anti-GTPase HRAS antibody [Y132] (ab32417) is a rabbit monoclonal antibody detecting GTPase HRAS in Western Blot, IP . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y132,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Reactivity with other RAS members has not been tested.

Product details:
What is this antibody validated in?
Anti-GTPase HRAS antibody [Y132] (ab32417) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-GTPase HRAS [Y132] (ab32417) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.
Reviewed by scientists
Anti-GTPase HRAS [Y132] (ab32417) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-GTPase HRAS antibody [Y132] (ab32417) has been confirmed by Western blot testing in HRAS Knockout HEK-293 cells.
Other related products
We have a range of other formats of antibody clone [Y132] also available for your convenience: ab32417, Carrier free -
ab239815
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GTPase HRAS also known as H-RAS or HRAS1 is a small GTPase enzyme involved in intracellular signal transduction. It has a molecular mass of approximately 21 kDa. HRAS is widely expressed in various tissues and plays a role in regulating cell growth and differentiation. The HRAS protein functions as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. This activity is facilitated by the HRAS protein's intrinsic GTPase activity which hydrolyzes GTP to GDP.
Biological function summary
GTPase HRAS participates in the regulation of cell proliferation differentiation and survival serving as an essential component of several signal transduction complexes. HRAS interacts with various signaling molecules and pathways to mediate mitogenic signals. Through these interactions the HRAS protein modulates cellular responses to extracellular stimuli influencing processes like growth and division playing an important role in maintaining normal cellular functions.
Pathways
The involvement of GTPase HRAS in the MAPK/ERK and the PI3K/AKT pathways illustrates its importance in transmitting signals from cell surface receptors. These pathways control a variety of cellular functions including growth survival and metabolism. HRAS interacts with related proteins like KRAS and NRAS integral members of the RAS family. These interactions highlight its role in signaling cascades that contribute to the cellular response to growth factors.
Mutations and aberrant activation of GTPase HRAS link to oncogenic processes particularly in bladder and thyroid cancers. The mutated HRAS protein contributes to the unchecked cellular proliferation seen in tumorigenesis. Additionally HRAS mutations associate with developmental disorders such as Costello syndrome where aberrant signaling disrupts normal developmental processes. HRAS-related pathways often involve other RAS family members like KRAS underlining their collective impact in disease states.