Abcam, ab324293, Anti-SIRT1 antibody [19A7AB4] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal SIRT1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:19A7AB4,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Expression levels of the target protein vary with sample type and some optimisation may be required. For western blotting, more concentrated lysates may be required when using tissues samples.

Product details:
ab324293 is the carrier-free version of
ab110304
What is this antibody validated in?
Anti-SIRT1 antibody [19A7AB4] (
ab110304
) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of SIRT1?
Anti-SIRT1 [19A7AB4] (
ab110304
) specifically detects a band for SIRT1 (UniProt: Q96EB6) at a molecular weight of 81kDa.
Trusted by the scientific community
Anti-SIRT1 [19A7AB4] (
ab110304
) was first used in a scientific publication in 2011 and has been cited over 310 times in peer-reviewed journals.
Reviewed by scientists
Anti-SIRT1 [19A7AB4] (
ab110304
) has over 25 independent reviews from customers.
Specificity confirmed
The specificity of Anti-SIRT1 antibody [19A7AB4] (
ab110304
) has been confirmed by Western blot testing in SIRT1 Knockout HEK-293 cells.
Other related products
We have a range of other formats of antibody clone [19A7AB4] also available for your convenience:
ab110304
, Alexa Fluor® 488 -
ab157401
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-ab110304 was produced in vitro using hybridomas grown in serum-free medium. Purity: >95% by SDS-PAGE., Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIRT1 also known as Silent mating type information regulation 2 homolog 1 is a NAD-dependent deacetylase enzyme. SIRT1 weighs approximately 120 kDa and plays an important role in regulating transcription apoptosis and stress resistance. Researchers have found SIRT1 in various tissues with higher expression in the heart brain and skeletal muscle. It is a component of the larger family of sirtuins which are involved in metabolic regulation and aging.
Biological function summary
SIRT1 modulates several cellular processes such as gene silencing DNA repair and lifespan extension. SIRT1 participates in complexes with other proteins including histones and transcription factors to influence chromatin structure and gene expression. It acts through deacetylation of target proteins affecting their function and stability. The activity of SIRT1 is also linked to environmental and cellular conditions including caloric intake and oxidative stress.
Pathways
SIRT1 is integral in the regulation of metabolic and longevity pathways. It interacts with the FOXO family proteins and the tumor suppressor protein p53 aiding in response to cellular stress and metabolic demands. The role of SIRT1 in the insulin signaling pathway exemplifies its influence on glucose homeostasis and energy balance. These interactions highlight its importance in metabolic health and aging.
SIRT1 links to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like type 2 diabetes. In Alzheimer's disease SIRT1 interacts with the amyloid precursor protein suggesting a protective role against amyloid-beta accumulation. Additionally studies have shown connections between SIRT1 and insulin receptor substrates highlighting its role in managing insulin sensitivity and glucose metabolism in diabetes. Understanding SIRT1's functions offers potential therapeutic targets for these disorders.