Abcam, ab32516, Anti-AIF antibody [E20] - Mitochondrial Marker

Size: 20µL / 100µL / 1mL
Anti-AIF antibody [E20] - Mitochondrial Marker (ab32516) is a rabbit monoclonal antibody detecting AIF in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 70 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E20,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:WB, ICC/IF, Flow Cyt (Intra), IHC-P, IHC-Fr, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-AIF antibody [E20] - Mitochondrial Marker (ab32516) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of AIF?
Anti-AIF [E20] - Mitochondrial Marker (ab32516) specifically detects a band for AIF (UniProt: O95831) at a molecular weight of 67kDa.
Trusted by the scientific community
Anti-AIF [E20] - Mitochondrial Marker (ab32516) was first used in a scientific publication in 2006 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-AIF [E20] - Mitochondrial Marker (ab32516) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-AIF antibody [E20] - Mitochondrial Marker (ab32516) has been confirmed by Western blot testing in AIFM1 Knockout HEK-293 cells.
Other related products
We have a range of other formats of antibody clone [E20] also available for your convenience: ab32516, Alexa Fluor® 647 -
ab196847
, Biotin -
ab197526
, Carrier free -
ab220215
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AIF short for Apoptosis-Inducing Factor is a flavoprotein characterized by its mass of approximately 57 kDa. It resides in the mitochondria of cells where it plays a decisive role in apoptosis. Under normal conditions AIF remains in the mitochondrial intermembrane space but when cells receive apoptotic signals AIF translocates to the nucleus leading to chromatin condensation and large-scale DNA fragmentation. The protein is also known as AIFM1 and performs functions beyond apoptosis including regulation of reactive oxygen species. Often analyzed using tools like a mitochondrial AB marker researchers use AIF as an important part of understanding cell death mechanisms.
Biological function summary
AIF functions as an important element in mitochondrial-mediated apoptosis. Once released from mitochondria AIF triggers a caspase-independent pathway of apoptosis making it distinct from other pathways where caspases are central. While not part of a larger protein complex AIF interacts closely with several mitochondrial and nuclear elements to execute its functions. Researchers often monitor AIF activity using apoptosis ELISA kits which help in precise detection and quantification of this protein within cellular systems.
Pathways
The presence and activity of AIF align with the broader apoptotic pathway and mitochondrial respiration. AIF links significantly with other molecules like cytochrome c released during apoptosis although cytochrome c follows a caspase-dependent pathway. It also shows involvement in various cellular injury and stress response pathways reinforcing its role as a major player in cellular fate decisions and intrinsic apoptotic mechanisms.
AIF holds relevance in neurodegenerative disorders such as Parkinson's disease and certain forms of cancer. Misregulation or mutations in AIF can lead to increased susceptibility to these conditions highlighting its importance in maintaining normal cellular and mitochondrial function. AIF also interacts with other proteins implicated in these disorders such as E2F1 in cancer which shares similar apoptotic regulatory roles. These interactions highlight the biological significance of AIF beyond its fundamental apoptosis function making it a potential target for therapeutic interventions.