Abcam, ab32519, Anti-Myosin Phosphatase + Myosin Phosphatase 2 antibody [YE336]

Size: 100µL
Rabbit Monoclonal Myosin Phosphatase antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 12 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:YE336,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for human Myosin Phosphatase 1 and Myosin Phosphatase 2.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myosin Phosphatase also known as Myosin Light Chain Phosphatase is a critical enzyme involved in muscle contraction regulation. It has no specific subunit weight assigned to it but the holoenzyme usually consists of three subunits: a catalytic subunit (PP1c) a large regulatory subunit (MYPT1) and a small regulatory subunit (M20). Myosin Phosphatase is expressed in smooth muscle and non-muscle tissues where it plays a role in the relaxation phase of muscle contraction.
Biological function summary
Myosin Phosphatase functions by dephosphorylating myosin light chains which leads to muscle relaxation. It is an essential component of the Myosin Phosphatase holoenzyme complex which ensures smooth muscle function. Through its activity this enzyme influences processes such as cell motility cell division and vascular tone maintenance. Its activity is regulated by signals that affect the phosphorylation state of its target substrates.
Pathways
Myosin Phosphatase is a critical player in the Rho/Rhokinase signaling pathways. It participates in the regulation of cellular contraction and cytoskeletal dynamics. Within these pathways it interacts closely with Rho-associated protein kinase (ROCK) which can inhibit Myosin Phosphatase activity by phosphorylating the MYPT1 subunit. This pathway influence extends to its interactions with other proteins like Cdc42 and Rac which participate in similar cellular functions.
Myosin Phosphatase is linked to hypertension and asthma. Dysregulation of Myosin Phosphatase activity can result in altered muscle tone and has implications in abnormal vascular smooth muscle contraction contributing to high blood pressure. In asthma abnormal airway smooth muscle contractions lead to bronchoconstriction further supported by its interaction with proteins such as ROCK and myosin light chain kinase (MLCK) both involved in similar disease-related pathways.