Abcam, ab325280, AZdye 568 Anti-GFP antibody [1H1 / 1B2] - FluoTag®-X4

Size: 100µL
Fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates featuring FluoTag® technology.
Key facts
Host species:Alpaca,
Clonality:Multiclonal,
Clone number:1H1 / 1B2,
Isotype:IgG,
Conjugation:AZdye 568,
Excitation/Emission:Ex: 578nm, Em: 602nm,
Carrier free:No,
Reacts with:Aequorea victoria,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
FluoTags®
FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.
Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.
FluoTag®-X4 (One target - Two sdAb – Four fluorophores (1:2:4)
Each FluoTag®-X4 reagent is a blend of two different sdAbs (FluoTag®-X2 and FluoTag®-X2-B) molecules that independently bind the target protein. As each sdAb carries two fluorophores per protein molecule, the blend can direct up to 4 fluorophores to each target protein. The reagent is therefore up to 2-fold brighter than the corresponding FluoTag®-X2 products.
The maximal displacement of fluorophores from each antigen-binding surface is ~3 nm. FluoTag®-X4 reagents have the same size as the corresponding FluoTag®-X2 products and therefore show similarly good tissue penetration features.
Features:
A blend of two high affinity monovalent sdAbs directs four fluorophores to each target protein – ideal for bright signals
Small size for optimal access to the respective target protein, also in crowded environment or 3D samples

Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1 month, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle