Abcam, ab32536, Anti-NF-kB p65 antibody [E379]

Size: 20µL / 100µL / 1mL
Anti-NF-kB p65 antibody [E379] (ab32536) is a rabbit monoclonal antibody detecting NF-kB p65 in Western Blot, IP, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 430 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E379,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognises NF-kB p65. For WB, this antibody is unsuitable for detecting NF-KB p65 in mouse tissue lysates. The expression of NF-KB p65 is increased by lipopolysaccharides treatment reported by PMID: 18036230. Although some papers support the expression of NF-kB p65 in mouse tissue (PMID: 21479220 and 20008488), This antibody cannot detect band of interest in these mouse tissue.

Product details:
Abcam recommended secondaries - Goat Anti-Rabbit HRP (
ab205718
) and Goat Anti-Rabbit Alexa Fluor® 488 (
ab150077
). Or search our wide range of secondary antibodies for use with your experiment.
Product Specifications
Anti-NF-kB p65 antibody [E379] (ab32536) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse samples.
Anti-NF-kB p65 antibody [E379] (ab32536) specifically detects NF-kB p65 (UniProt ID: Q04206; Molecular weight: 60kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-NF-kB p65 antibody [E379] (ab32536) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-NF-kB p65 antibody [E379] (ab32536) has been confirmed by testing in knockout samples.
Anti-NF-kB p65 antibody [E379] (ab32536) has been cited over 439 times in peer reviewed journals and is trusted by the scientific community.
Anti-NF-kB p65 antibody [E379] (ab32536) has 15 independent reviews from customers.
Related Products
Antibody clone E379 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Biotin, PE, Alexa Fluor® 594 (
ab190205
ab190589
ab205823
ab208750
ab214846
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle