Abcam, ab32560, Anti-14-3-3 alpha + beta antibody [Y62]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal 1433B phospho S184 + S186 antibody. Suitable for IP, Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human, Mouse, Recombinant full length protein - Human, Rat samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y62,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
14-3-3 alpha + beta proteins also known as YWHAZ (14-3-3 zeta) and YWHAB (14-3-3 beta) are part of a family of conserved proteins weighing approximately 28-33 kDa each. These proteins are found in many tissues and engage in binding phosphorylated serine/threonine residues on their target molecules. This binding influences the activity localization and stability of these target proteins. Expression of 14-3-3 alpha + beta proteins is prominent in the brain where they participate in key signaling pathways.
Biological function summary
14-3-3 proteins play central roles in regulating diverse cellular processes. These proteins frequently serve as scaffolding molecules stabilizing complexes for signal transduction pathways. By interacting with numerous partner proteins 14-3-3 complexes modulate cell cycle control apoptosis and DNA damage response. They help maintain cellular homeostasis by binding and regulating enzymes receptors and transcription factors.
Pathways
14-3-3 proteins are integral to many signaling cascades. The MAPK/ERK pathway involves these proteins with 14-3-3 binding to Raf kinases influencing signal transduction. They also participate in the PI3K/Akt pathway where 14-3-3 interaction with Akt affects cell survival and growth. 14-3-3 proteins regulate various cellular functions by interacting with related kinases and phosphatases in these pathways.
Alterations of 14-3-3 protein function have significant implications. Neurodegenerative disorders such as Alzheimer’s disease show changes in 14-3-3 protein binding patterns affecting neuronal stability. Aberrant 14-3-3 interactions also connect to cancerous pathways particularly in breast cancer where regulation of p53 and other tumor suppressor proteins becomes disrupted. 14-3-3 proteins influence disease progression through their extensive network of protein interactions.