Abcam, ab32566, Anti-DNA PKcs antibody [Y393]

Size: 100µL / 1mL
Anti-DNA PKcs antibody [Y393] (ab32566) is a rabbit monoclonal antibody detecting DNA PKcs in Western Blot, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y393,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ChIC/CUT&RUN-seq, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for DNA PKcs. It may also detect the splice isoform 2.Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

Product details:
What is this antibody validated in?
Anti-DNA PKcs antibody [Y393] (ab32566) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of DNA PKcs?
Anti-DNA PKcs [Y393] (ab32566) specifically detects a band for DNA PKcs (UniProt: P78527) at a molecular weight of 469kDa.
Trusted by the scientific community
Anti-DNA PKcs [Y393] (ab32566) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-DNA PKcs [Y393] (ab32566) has over 10 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-DNA PKcs antibody [Y393] (ab32566) has been confirmed by Western blot testing in PRKDC Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [Y393] also available for your convenience: ab32566, Carrier free -
ab174575
, HRP -
ab195537
, Alexa Fluor® 488 -
ab311006
, Alexa Fluor® 647 -
ab311134
, Alexa Fluor® 594 -
ab311768
, Alexa Fluor® 568 -
ab313049
, Alexa Fluor® 555 -
ab313250
, Alexa Fluor® 750 -
ab321610
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DNA-dependent protein kinase catalytic subunit often abbreviated as DNA-PKcs or PKcs is an important component in the cellular machinery responsible for DNA repair. This protein weighs approximately 470 kDa and is predominantly expressed in various tissue types with higher levels found in lymphoid tissues. The DNA-PKcs functions as a serine/threonine protein kinase and becomes active upon binding to DNA contributing to its role in maintaining genome integrity.
Biological function summary
DNA-PKcs plays an essential role in the non-homologous end joining (NHEJ) repair pathway which repairs double-strand breaks in DNA. DNA-PKcs forms a complex with the Ku70/80 heterodimer serving as a critical component of the DNA repair mechanism. This complex recognizes DNA ends facilitates their synapsis and activates other enzymes involved in ligating the broken DNA strands therefore promoting cellular survival following DNA damage.
Pathways
DNA-PKcs deeply integrates into the DNA damage response pathway. It collaborates closely with proteins such as ATM (ataxia-telangiectasia mutated) to sense DNA damage and initiate repair. DNA-PKcs is involved in V(D)J recombination vital for the generation of diverse antibodies in immune cells. Its activity is essential for coordinating with other proteins including XRCC4 and Ligase IV to ensure efficient DNA repair processes are executed.
Defects in DNA-PKcs are linked to severe combined immunodeficiency (SCID) due to its central role in V(D)J recombination. Dysfunction in its pathway also relates to cancer where impaired DNA repair mechanisms substantially increase genomic instability leading to tumorigenesis. DNA-PKcs interacts with other proteins like BRCA1 and p53 in tumors where its overexpression or mutations can contribute to resistance against radiation and chemotherapeutic agents. These interactions highlight the importance of DNA-PK inhibitors as potential therapeutic strategies in oncology.