Abcam, ab32575, Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

Size: 100µL / 1mL
Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab32575) is a rabbit monoclonal antibody detecting ACTG2 in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 580 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E184,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-P, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:For immunohistochemistry, this antibody only detects actin in smooth muscle and not cardiac muscle.This antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.

Product details:
Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab32575) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB in human, mouse, rat samples.
Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab32575) has been cited over 584 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody (Anti-ACTG2 acetyl E3 antibody/ Anti-ACTA2 antibody/ Anti-ACTG2 antibody) [E184] (ab32575) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab32575) has 22 independent reviews from customers.
Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab32575) specifically detects alpha smooth muscle Actin; ACTG2 Acetyl-E3 (UniProt ID: P62736; Molecular weight: 42kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone E184 -
ab215368
Antibody clone E184 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, HRP, PE, Alexa Fluor® 405, FITC, APC (
ab196919
ab196920
ab209435
ab210128
ab223920
ab223921
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha smooth muscle Actin also known as ACTG2 is an important protein in muscle contraction and cellular structure. It is most commonly found in smooth muscle cells and is not typically expressed in striated muscle. This protein is part of the actin family and has a molecular mass of approximately 42 kDa. ACTG2 plays a role in forming microfilaments which are key components of the muscle contractile machinery. In smooth muscle tissues such as those found in the intestines and blood vessels ACTG2 is often detected together with other cytoskeletal and regulatory proteins.
Biological function summary
ACTG2 contributes to muscle contraction by facilitating the interaction between actin and myosin. It is not considered a part of a protein complex but interacts closely with several other proteins involved in contractile functions. Beyond contraction ACTG2 also supports cellular integrity and shape maintenance. In tissues ACTG2 is involved in activities that require dynamic structural reorganization such as during cellular migration and wound healing highlighting its versatility beyond just a contractile agent.
Pathways
ACTG2 participates in the regulation of the smooth muscle contraction pathway influencing muscle tone and responsiveness to stimuli. The actin-myosin interaction where ACTG2 is involved is critical for the contractile process with other proteins like myosin light chain kinase (MLCK) and tropomyosin playing supportive roles. Additionally the integrin signaling pathway is another context where ACTG2 is indirectly involved aiding in the transmission of mechanical signals from the extracellular matrix to the cell.
ACTG2 is associated with conditions such as megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS) and various vascular disorders. Mutations in the ACTG2 gene can disrupt normal muscle function leading to defective peristalsis in the gastrointestinal tract as seen in MMIHS. The connection between ACTG2 and myosin regulatory light chains illustrates how changes in its function can impact muscle contractility and lead to disease. In vascular disorders abnormal expression of ACTG2 is often linked to impaired vessel tone and blood flow regulation.