Abcam, ab3317, Anti-Proteasome 19S S4/PSMC1 antibody

Size: 100µL
Rabbit Polyclonal Proteasome 19S S4/PSMC1 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Full Length Protein corresponding to Human 26S proteasome regulatory subunit 4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Human 26S proteasome regulatory subunit 4. The exact immunogen used to generate this antibody is proprietary information.P62191,
Specificity:Detects proteasome 19S subunit S4.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-Constituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Proteasome 19S S4 also known as PSMC1 is a mechanical component of the 26S proteasome an ATP-dependent protease complex responsible for protein degradation. This protein functions as an ATPase and contributes to the unfolding of ubiquitinated proteins preparing them for translocation into the 20S core particle of the proteasome for degradation. S4/PSMC1 has a molecular mass of approximately 49 kDa and is expressed ubiquitously across various cell types reflecting its essential role in cellular protein homeostasis.
Biological function summary
PSMC1 functions as an integral part of the 19S regulatory particle of the 26S proteasome complex. This complex facilitates the recognition unfolding and translocation of polyubiquitinated proteins into the proteolytic chamber of the 20S core particle for degradation. This activity plays a significant role in regulating intracellular protein concentrations thereby controlling processes like cell cycle progression apoptosis and responses to oxidative stress. PSMC1’s activity ensures a clean cellular environment by removing damaged or nonfunctional proteins.
Pathways
Functions of PSMC1 integrate into the ubiquitin-proteasome pathway and the cell cycle control pathway. In the ubiquitin-proteasome pathway PSMC1 collaborates with other ATPase subunits and regulatory proteins helping to maintain protein degradation balance and works closely with ubiquitin-conjugating enzymes responsible for tagging proteins for degradation. In the cell cycle control pathway the degradation of cyclins and cyclin-dependent kinase inhibitors by the proteasome including PSMC1 regulates cell cycle transitions and ensures proper cell division and function.
Alterations in PSMC1 function or expression can link to disorders such as cancer and neurodegenerative diseases. In cancer the dysregulation of PSMC1 can affect cellular proliferation by disrupting regulated protein degradation leading to uncontrolled cell growth. In neurodegenerative diseases such as Parkinson's impaired proteasome function including the activity of PSMC1 may lead to the accumulation of misfolded proteins contributing to neuronal cell death. Connections to other proteins like cyclins in cancer or alpha-synuclein in Parkinson's enhance the consequences of dysfunctional PSMC1 activity in these diseases.