Abcam, ab34549, Anti-Trypsin Inhibitor antibody

Size: 100µg
Rabbit Polyclonal Trypsin Inhibitor antibody. Suitable for ELISA, Dot, WB, Conjugation and reacts with Soybean samples. Cited in 5 publications. Immunogen corresponding to Full Length Protein corresponding to Soybean KTI3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Soybean,
Applications:Dot, WB, Conjugation, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Soybean KTI3. The exact immunogen used to generate this antibody is proprietary information.P01070

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification technique-Ion exchange chromatography, Purification notes-Anti-trypsin inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer., Storage buffer-pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Trypsin Inhibitor also known as Serine Protease Inhibitor Kazal-Type 1 (SPINK1) is a small protein with a mass of around 6 kilodaltons. It inhibits the activity of trypsin a serine protease by forming a stable complex which prevents the breakdown of protein substrates. This inhibitor is primarily expressed in pancreatic tissues as well as throughout the gastrointestinal tract. Its function is important in regulating the activity of digestive enzymes to prevent autodigestion of pancreatic cells.
Biological function summary
Trypsin Inhibitor plays a role in maintaining the balance of proteolytic activity in the pancreas. Inhibition of trypsin serves to protect tissue integrity by preventing premature activation of digestive enzymes. SPINK1 is not part of a larger protein complex but functions independently to bind with trypsin and other related proteases. This modulation of protease activity is essential for normal cellular homeostasis and digestive processes.
Pathways
Trypsin Inhibitor takes part in the proteolytic cascade that occurs in pancreatic cells. It acts within the balance-maintaining pathway between trypsinogen and trypsin where it inhibits premature conversion and activation of these enzymes. Related proteins include chymotrypsin and elastase which like trypsin are activated from their precursors in the digestive process. The Trypsin Inhibitor therefore ensures the safe regulation of these proteolytic enzymes.
Trypsin Inhibitor appears connected to pancreatitis and certain forms of pancreatic cancer. Mutations in the SPINK1 gene are associated with chronic pancreatitis where the regulated process of enzyme inhibition is disrupted leading to pancreatic tissue damage. Additionally overexpression of Trypsin Inhibitor has been noted in some pancreatic cancers potentially aiding in tumor progression by preventing enzyme-mediated destruction. The protein interacts with mutated forms of trypsin that may contribute to these disorders.