Abcam, ab34593, Anti-LEFTY2 antibody [7C5G1H6H10]

Size: 100µg
Mouse Monoclonal LEFTY2 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Human LEFTY2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:7C5G1H6H10,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Protein within Human LEFTY2. The exact immunogen used to generate this antibody is proprietary information.O00292,
Specificity:This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.

Product details:
Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LEFTY2 also known as Left-Right Determination Factor 2 is a member of the transforming growth factor beta (TGF-β) superfamily. This protein has a molecular mass of approximately 38 kDa. LEFTY2 is expressed mainly in visceral endoderm and left side mesoderm tissues. The gene encoding LEFTY2 is found within the human chromosome 1. This protein plays a mechanical role in the regulation of cellular signaling and developmental processes.
Biological function summary
LEFTY2 functions as an important regulator in ensuring proper left-right asymmetry during embryonic development. It is a monomeric protein that is not part of a larger complex. LEFTY2 contributes to the modulation of Nodal signaling inhibiting excessive activity that can disrupt normal development. This modulation ensures the establishment of asymmetric organ positioning and plays an important role in maintaining developmental balance in the body.
Pathways
LEFTY2 integrates into the Nodal signaling pathway and the Activin signaling pathway. The interaction with Nodal proteins narrows down Nodal signaling ensuring the accurate development of the left-right axis. LEFTY2 also relates functionally and antagonistically to other proteins like Nodal and Activin A balancing their signaling actions to regulate developmental processes.
LEFTY2 has a connection with asymmetry-related conditions such as situs inversus where organ placement within the body is mirrored from normal positions. Additionally research links irregular LEFTY2 expression to some cancer types including ovarian cancer. Through these disorders LEFTY2 interacts with proteins like Nodal which plays a role in cancer cell proliferation by influencing the signaling pathways that govern cellular growth and differentiation.